Patents by Inventor Beau Peelle

Methods and compositions comprising GFP

Patent number: 7528242
Abstract: The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (rGFP), and Ptilosarcus green fluorescent proteins (pGFP). In particular, the invention relates to the use of Renilla GFP or Ptilosarcus GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening libraries using rGFP or pGFP.
Type: Grant
Filed: July 8, 2005
Issued: May 5, 2009
Assignee: Rigel Pharmaceuticals, Inc.
Inventors: David Anderson, Beau Peelle


Patent number: 7488593
Abstract: One-dimensional ring structures form M13 viruses were constructed by two genetic modifications encoding binding peptides and synthesis of a heterobifunctional linker molecule. The bifunctional viruses displayed an anti-streptavidin peptide and hexahistidine (SEQ ID NO: 4) peptide at opposite ends of the virus as pIII and pIX fusions. Stoichiometic addition of the streptavidin-NiNTA linker molecule led to the reversible formation of virus-based nanorings with circumferences corresponding to lengths of the packagable DNAs. These virus-based ring structures can be further engineered to nucleate inorganic materials and form metallic, magnetic, or semiconductor nanorings using trifunctionalized viruses.
Type: Grant
Filed: October 15, 2004
Issued: February 10, 2009
Assignees: Board of Regents, The University of Texas System, Massachusetts Institute of Technology
Inventors: Angela M. Belcher, Beau Peelle, Ki Tae Nam

 

Application number: 20070298973
Abstract: The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (rGFP), and Ptilosarcus green fluorescent proteins (pGFP). In particular, the invention relates to the use of Renilla GFP or Ptilosarcus GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening libraries using rGFP or pGFP.
Type: Application
Filed: July 8, 2005
Issued: December 27, 2007
Inventors: David Anderson, Beau Peelle
Patent number: 7193052
Abstract: The present invention relates to directed protein evolution in mammalian cells and improved mutants of Discosoma sp. red fluorescent proteins.
Type: Grant
Filed: December 17, 2003
Issued: March 20, 2007
Assignees: Rigel Pharmaceuticals, Incorporated, Becton, Dickinson and Company
Inventor: Beau Peelle
Patent number: 7090976
Abstract: The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (rGFP), and Ptilosarcus green fluorescent proteins (pGFP). In particular, the invention relates to the use of Renilla GFP or Ptilosarcus GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening libraries using rGFP or pGFP.
Type: Grant
Filed: April 24, 2002
Issued: August 15, 2006
Assignee: Rigel Pharmaceuticals, Inc.
Inventors: David Anderson, Beau Peelle
Application number: 20060003387
Abstract: Eukaryotic cell display libraries for use in panning processes comprising expressed biomolecules for specific and selective binding and enrichment to solid material surfaces including, for example, metal, magnetic, and semiconducting surfaces. Display can be regulated. Peptide and protein display on yeast cells are preferred. Solid materials can be fabricated in the presence of cell display libraries which have been subjected to panning against the solid materials. Nanoparticles can be grown in the presence of the biomolecules from reactive precursors. The nanoparticles can show quantum confinement effects. Self-healing films can be prepared.
Type: Application
Filed: February 7, 2005
Issued: January 5, 2006
Inventors: Beau Peelle, Angela Belcher, Karl Wittrup, Eric Krauland
Application number: 20050170336
Abstract: One-dimensional ring structures form M13 viruses were constructed by two genetic modifications encoding binding peptides and synthesis of a heterobifunctional linker molecule. The bifunctional viruses displayed an anti-streptavidin peptide and hexahistidine (SEO ID NO: 4) peptide at opposite ends of the virus as pIII and pIX fusions. Stoichiometic addition of the streptavidin-NiNTA linker molecule led to the reversible formation of virus-based nanorings with circumferences corresponding to lengths of the packagable DNAs. These virus-based ring structures can be further engineered to nucleate inorganic materials and form metallic, magnetic, or semiconductor nanorings using trifunctionalized viruses.
Type: Application
Filed: October 15, 2004
Issued: August 4, 2005
Inventors: Angela Belcher, Beau Peelle, Ki Nam
Application number: 20040126850
Abstract: The present invention relates to directed protein evolution in mammalian cells and improved mutants of Discosoma sp. red fluorescent proteins.
Type: Application
Filed: December 17, 2003
Issued: July 1, 2004
Assignees: Rigel Pharmaceuticals, Incorporated, Becton, Dickinson and Company
Inventor: Beau Peelle
Patent number: 6723537
Abstract: The present invention relates to directed protein evolution in mammalian cells and improved mutants of Discosoma sp. red fluorescent proteins.
Type: Grant
Filed: May 20, 2002
Issued: April 20, 2004
Assignees: Rigel Pharmaceuticals, Incorporated, Becton, Dickinson and Company
Inventor: Beau Peelle
Application number: 20030149254
Abstract: The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (rGFP), and Ptilosarcus green fluorescent proteins (pGFP). In particular, the invention relates to the use of Renilla GFP or Ptilosarcus GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening libraries using rGFP or pGFP.
Type: Application
Filed: April 24, 2002
Issued: August 7, 2003
Assignee: Rigel Pharmaceuticals, Inc.
Inventors: David Anderson, Beau Peelle
Application number: 20030077730
Abstract: The present invention relates to directed protein evolution in mammalian cells and improved mutants of Discosoma sp. red fluorescent proteins.
Type: Application
Filed: May 20, 2002
Issued: April 24, 2003
Assignee: Rigel Pharmaceuticals, Incorporated
Inventor: Beau Peelle